Author response image 7. Co-localization of green and red channels.
(A) co-localization of high intensity green and high intensity red image shows a yellow merge image. Histogram shows overlap of intensities. (B) Co-localization of low intensity green dot with bright green ring with a high intensity red dot, Merge image does not show yellow colour. Histogram shows high intensity of green channel at the ring and high intensity of red channel at the inside. (C) Green LAMP1 positive lysosome shows high intensity border and lower intensity lumen together with high intensity myelin in red. The merge image does not appear yellow.