Fig. 3.

Analysis by TLC of coated EOPC (A) and OEPC (B) hydrolysis revealed with UV light at 254 nm (left panels) and with 10% copper sulfate and 10% phosphoric acid in water followed by charring at 150°C for 15 min (right-hand panels). The standards (Std) used (10 μg of each compound) were α-eleostearic acid, EOPC, and OEPC. Coated PCs were hydrolyzed by GPL-RP₂ or ppPLA₂ (0.5 μg/well). The chromatographic solvent system was chloroform/methanol/water (65/35/4, v/v/v) and hexane/diethylether/acetic acid (84/16/1, v/v/v) containing 0.001% (w/v) BHT.