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. 2016 Aug;57(8):1435–1446. doi: 10.1194/jlr.M067215

Fig. 7.

Fig. 7.

STRA6 siRNA damages STRA6 cascades and induces renal cell injury. A: Western blots show LOX1, STRA6, CRBP1, RARα, RXRα, pJNK, pSmad2, and collagen 1 (Col1) in cell lysate of STRA6 siRNA (siSTRA6) or control siRNA (siC) transfected HK-2 cells cultured in PBS (Ctl)-, L1-, and L5-treated HK-2 cells for 24 h (n = 3). Quantitative analysis showed significance for the increase of LOX1/actin (B); the decrease of STRA6/actin (C), CRBP1/actin (D), RARα/actin (E), RXRα/actin (F), pJNK/JNK (G), pSmad2/Smad2 (H), and collagen 1/actin (I) in STRA6 siRNA-transfected HK-2 cells in Ctl and L1 groups. J: ELISA also showed a significant increase of TGFβ1 in culture medium of siSTRA6-transfected HK-2 cells in Ctl, L1, and L5 groups. K: Cytochemistry images show that STRA6 siRNA transfection increased the nuclei of apoptotic cells (green fluorescence) in Ctl- and L1-treated cells. All results are represented as mean ± SE. §P < 0.05 versus and L1-treated siC cells; *P < 0.05 versus Ctl-treated siC cells; #P < 0.05 versus L1-treated siC cells; +P < 0.05 versus L5-treated siC cells.