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. 2016 Aug;57(8):1507–1517. doi: 10.1194/jlr.M068874

TABLE 2.

ANS fluorescence in the presence of WT and elongation variants of apoA-I

Protein WMF (ANS) (nm)a IWTb IPBc
Δ(185-243) 488 (−29) 0.54 ± 0.09 2.14 ± 0.45
Δ(198-243) 488 (−29) 0.52 ± 0.08 2.06 ± 0.38
Δ(209-243) 490 (−27) 0.50 ± 0.08 2.01 ± 0.39
Δ(220-243) 489 (−28) 0.50 ± 0.08 1.99 ± 0.38
Δ(231-243) 488 (−29) 0.53 ± 0.06 2.11 ± 0.31
WT 477 (−40) 1.00 ± 0.06 3.98 ± 0.31
BSA 471 (−54) 1.78 ± 0.05 7.08 ± 0.23
ANS in PB alone 517 0.25 ± 0.04 1.00 ± 0.18

WMF, wavelength of maximum fluorescence; PB, phosphate buffer.

a

ANS fluorescence in the presence of 0.05 mg/ml WT or the elongation variants of apoA-I or BSA or in phosphate buffer alone. Values in parentheses show change in WMF compared with ANS fluorescence in phosphate buffer alone.

b

ANS fluorescence intensity in relative units compared with the fluorescence in the presence of WT apoA-I.

c

ANS fluorescence intensity in relative units compared with the fluorescence in the buffer alone.