TABLE 2.
ANS fluorescence in the presence of WT and elongation variants of apoA-I
| Protein | WMF (ANS) (nm)a | IWTb | IPBc |
| Δ(185-243) | 488 (−29) | 0.54 ± 0.09 | 2.14 ± 0.45 |
| Δ(198-243) | 488 (−29) | 0.52 ± 0.08 | 2.06 ± 0.38 |
| Δ(209-243) | 490 (−27) | 0.50 ± 0.08 | 2.01 ± 0.39 |
| Δ(220-243) | 489 (−28) | 0.50 ± 0.08 | 1.99 ± 0.38 |
| Δ(231-243) | 488 (−29) | 0.53 ± 0.06 | 2.11 ± 0.31 |
| WT | 477 (−40) | 1.00 ± 0.06 | 3.98 ± 0.31 |
| BSA | 471 (−54) | 1.78 ± 0.05 | 7.08 ± 0.23 |
| ANS in PB alone | 517 | 0.25 ± 0.04 | 1.00 ± 0.18 |
WMF, wavelength of maximum fluorescence; PB, phosphate buffer.
a
ANS fluorescence in the presence of 0.05 mg/ml WT or the elongation variants of apoA-I or BSA or in phosphate buffer alone. Values in parentheses show change in WMF compared with ANS fluorescence in phosphate buffer alone.
b
ANS fluorescence intensity in relative units compared with the fluorescence in the presence of WT apoA-I.
c
ANS fluorescence intensity in relative units compared with the fluorescence in the buffer alone.