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. 2016 Aug;57(8):1518–1528. doi: 10.1194/jlr.M068981

Fig. 3.

Fig. 3.

MS/MS and MS3 spectra of the α-ketol (8-hydroxy-9-oxo-12Z-octadecenoic acid) formed from 18:2n-6 and [13C18]18:2n-6 by recombinant EAS28473 (8R-DOX-AOS). A: MS/MS spectrum of 8-hydroxy-9-oxo-12Z-octadecenoic acid. B: MS/MS spectrum of the monodeuterated α-ketol, [11S-2H]8-hydroxy-9-oxo-12Z-octadecenoic acid. C: MS3 spectrum of 8-hydroxy-9-oxo-12Z-octadecenoic acid. D: MS3 spectrum of [13C18]8-hydroxy-9-oxo-12Z-octadecenoic acid. E: MS3 spectrum of [13C18]8-hydroxy-9-oxo-12Z-octadecenoic acid. The number of carbon atoms of some fragment ions is marked Csubscript in (E). Important fragments are labeled in a larger font and colored. NL, intensity normalized to 100%. The ions at m/z 282 in (D) and (E), which are likely formed by loss of 47 (13CO+water) and 29 (13CO), respectively, are marked *.