Fig. 6.

The 13S-LOX and 5,8-LDS activities of mycelia and an overview of fatty acid DOXs of Z. tritici. A: RP-HPLC-MS/MS analysis of hydroperoxides after reduction to alcohols. The inset shows that 13S-HODE was the main product (top) as judged from the separation of ¹³C-labeled 13R- and 13S-HODE (bottom). B: Phylogenetic tree of five iron LOXs. The GenBank identification numbers are (from top to bottom): EGP90986, EJT77580, EAL84806, EKX38530, and BAI99788. The phylogenetic tree was constructed with MEGA6. The percent sequence identity with EGP90986 is indicated. C: Overview of the oxidation of 18:2n-6 by mycelia (left) and by two recombinant DOX-AOSs (right) of Z. tritici.