Fig. 4.

Cl₂ gas exposure increases chlorinated fatty acids, GSH-fattyaldehyde adducts, and chlorotyrosine in the circulation. C57bl/6 mice were exposed to Cl₂ gas (400 ppm, 30 min) and then brought back to room air. At the indicated times thereafter, plasma was collected and free (black bars) and esterified (gray bars) 2-Cl-PA (A) and 2-Cl-SA (B) were measured. Air indicates basal or air-exposed mice. Time point 0 indicates immediately post-Cl₂ exposure. Data shown are mean ± SEM, n = 3–4 (except 72 h, n = 2). *P < 0.05 by one-way ANOVA with Dunnett’s multiple comparison post test relative to air. ^#P < 0.05 by two-way repeated measures-ANOVA with Bonferroni post test comparing free and esterified levels at each time. C: GSH adducts with palmitaldehyde (black bars) and stearaldehyde (open bars) in the plasma. Data shown are mean ± SEM, n = 3–4 (except 72 h, n = 2). *P < 0.05 by one-way ANOVA with Dunnett’s multiple comparison post test relative to air. ^#P < 0.05 by two-way repeated measures-ANOVA with Bonferroni post test comparing palmitaldehyde- and stearaldehyde-GSH adducts at each time. D: The 3-Cl-Tyr levels normalized to total tyrosine in plasma. Maximum values at 2 h correspond to 0.1 ± 0.01 pmol/μl plasma. All data are mean ± SEM, n = 3–4. *P < 0.05 by one-way ANOVA with Dunnett’s multiple comparison post test relative to air.