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. 2016 Aug;57(8):1552–1563. doi: 10.1194/jlr.M069229

TABLE 2.

Substrate binding parameters calculated from the spectral response of human CYP51 to the binding of lanosterol and obtusifoliol

Substrate ΔAmax Kd (μM) ΔAmax/Kda
Absorbance Units/μM P450/cm Low-to-High Spin Transition (%)
Lanosterol 0.029 ± 0.01 26 0.82 ± 0.13 32
Obtusifoliol 0.056 ± 0.01 51 0.99 ± 0.08 52
a

Apparent binding efficiency.