Table II.
Ability of SCD biochip in differentiating between hemoglobin phenotypes based on RBC adhesion to FN and LN microchannels
| FN |
LN |
|||||
|---|---|---|---|---|---|---|
| SS-AA | SC-AA | SS-SC | SS-AA | SC-AA | SS-SC | |
| Threshold | 9 | 9 | 30 | 16 | 16 | 170 |
| Sensitivity | 0.93 | 0.80 | 0.75 | 0.93 | 0.89 | 0.87 |
| Specificity | 1.00 | 1.00 | 0.70 | 1.00 | 1.00 | 0.78 |
| PLR | Inf | Inf | 2.50 | Inf | Inf | 3.90 |
| NLR | 0.07 | 0.20 | 0.36 | 0.07 | 0.11 | 0.17 |
| PPV | 1.00 | 1.00 | 0.88 | 1.00 | 1.00 | 0.87 |
| NPV | 0.83 | 0.83 | 0.50 | 0.89 | 0.89 | 0.78 |
| Accuracy | 0.95 | 0.90 | 0.74 | 0.96 | 0.94 | 0.83 |
| AUC | 0.88 | 0.88 | 0.77 | 0.86 | 0.99 | 0.89 |
Abbreviations: AUC, Area under the receiver operating characteristic curve; FN, fibronectin; LN, laminin; PLR, positive likelihood ratio; PPV, positive predictive value; NLR, negative likelihood ratio; NPV, negative predictive value.
Threshold unit is number of adhered RBCs per unit area.