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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Br J Haematol. 2016 Apr 12;174(3):425–436. doi: 10.1111/bjh.14075

Figure 5. Bone Marrow Stromal Cell (BMSC) co-culture overcomes drug-induced cytotoxicity of B-ALL cell line and CXCR4 deletion reverses this effect.

Figure 5

(A) Dot plots show representative experiment depicting viability of dexamethasone (DEX) treated NALM6 cells in the presence and absence of BMSC co-culture. In the NALM6-BMSC co-culture sample, viability of NALM6 cells in supernatant and those that migrated beneath the stroma was measured separately. (B) Bar graphs show mean of three separate experiments illustrated in dot plots. In addition to DEX treatment, the experiment was also performed with 2.5 μM 4-HydroperoxyCyclophosphamide (4HC) and 2 nM vincristine (VIN) in the presence and absence (control) of BMSC co-culture. Bar diagrams represent mean drug induced cytotoxicity (± SEM) of three separate experiments. Viability was measured using PI/DiOC6 staining at 48 h; asterisks indicate significant differences in cytotoxicity (*p < 0.05; **p < 0.01).