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. Author manuscript; available in PMC: 2016 Sep 22.
Published in final edited form as: Oncogene. 2016 Jan 25;35(35):4633–4640. doi: 10.1038/onc.2015.527

Figure 2. WASF3 is essential for NRG-induced EMT and invasion in HER2-positive breast cancer cells.

Figure 2

Transwell invasion assays demonstrate that NRG-treated SKBR3 cells show increased invasion (a) which is suppressed following treatment with 17-AAG. When WASF3 is knocked down in these cells (shW3-1 and shW3-2), NRG treatment no longer leads to increased invasion (b) compared with knockdown control cells (shGFP). NRG facilitates transition of epithelial SKBR3 cells to a mesenchymal phenotype, but this change is suppressed when WASF3 is knocked down (c). NRG treatment leads to increased ZEB1 levels in cells expressing the control shRNA (shGFP) but not in WASF3 knockdown cells (shW3). ** p<0.01; Student’s t-test.