Isolation and culture of adult fibrobalsts and AFS cells. a Primary explants outgrowth from ear skin tissue, b Subcultured fibroblast cells having 70–80 % confluence, c Confluent monolayer of subcultured cells of fibroblast, d Primary culture of AF cells on the day of seeding, e Attached cells with non-fibroblastic epithelial cell like phenotype, f spindle-shaped fibroblastic-like AFS cells (200 µm)