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. 2014 Dec 13;68(4):593–608. doi: 10.1007/s10616-014-9805-1

Fig. 1.

Fig. 1

Isolation and culture of adult fibrobalsts and AFS cells. a Primary explants outgrowth from ear skin tissue, b Subcultured fibroblast cells having 70–80 % confluence, c Confluent monolayer of subcultured cells of fibroblast, d Primary culture of AF cells on the day of seeding, e Attached cells with non-fibroblastic epithelial cell like phenotype, f spindle-shaped fibroblastic-like AFS cells (200 µm)