Table 2.
Cell counts of the MEFs at different passages
| Passage* | Day1 | Day2 | Day3 | Day4 | Day5 | Day6 | Day7 |
|---|---|---|---|---|---|---|---|
| 2 | 19,587.5 ± 697.4 | 25,068.3 ± 3,232.6 | 38,212.3 ± 12,254.0 | 54,597.0 ± 19,430.6 | 72,450.3 ± 28,540.8 | 81,612.8 ± 34,182.5 | 82,375.5 ± 34,478.4 |
| 3 | 19,626.8 ± 657.2 | 25,186.8 ± 2,109.5 | 35,667.5 ± 6,854.5 | 50,817.0 ± 11,734.7 | 66,143.8 ± 10,924.4 | 75,229.0 ± 12,321.6 | 75,579.3 ± 12,488.5 |
| 4 | 19,882.3 ± 243.9 | 23,898.7 ± 1,112.8 | 35,109.3 ± 2,360.1 | 48,890.7 ± 8,514.5 | 59,813.3 ± 14,212.6 | 70,101.5 ± 18,058.4 | 70,439.8 ± 18,066.2 |
| 5 | 20,069.7 ± 695.4 | 24,209.5 ± 1,945.8 | 32,542.7 ± 6,121.1 | 45,837.2 ± 11,215.9 | 57,695.0 ± 14,679.6 | 63,262.0 ± 17,267.8 | 63,058.7 ± 17,252.3 |
| 6 | 20,413.5 ± 526.1 | 22,769.8 ± 1,989.1 | 27,610.8 ± 2,584.2 | 35,871.8 ± 5,177.3 | 40,036.7 ± 6,741.8 | 43,987.7 ± 8,168.6 | 42,689.5 ± 8,074.1 |
| 7 | 19,530.2 ± 565.3 | 23,478.0 ± 414.1 | 27,014.0 ± 1,669.2 | 30,928.0 ± 1,307.6 | 36,200.0 ± 3,187.0 | 35,071.0 ± 2,762.0 | 33,897.7 ± 3,420.0 |
Day1 represents the cell numbers per well of 12-well plates, which were counted manually just after the seeding of cells. Day2 means the corresponding data read manually on the 2nd day, and so on. Differences were considered significant in the case of P < 0.05
* P < 0.01 among different passages