Table 4.
Cell Counts of the MEFs inactivated by irradiation
| Dose (Gy)* | Dose rate (Gy/min)#,& | Passage^ | Day1 | Day3 | Day5 | Day7 |
|---|---|---|---|---|---|---|
| 15 | 1.0 | 3 | 20,073.5 ± 945.4 | 27,792.5 ± 4,085.0 | 32,189.5 ± 5,530.3 | 35,084.5 ± 6,507.5 |
| 4 | 19,852.0 ± 714.2 | 29,331.0 ± 323.9 | 34,171.5 ± 88.4 | 35,753.5 ± 232.6 | ||
| 5 | 19,551.5 ± 591.8 | 23,560.0 ± 2,199.1 | 24,987.0 ± 1,776.3 | 26,598.5 ± 2,684.9 | ||
| 1.5 | 3 | 20,896.5 ± 37.5 | 25,581.5 ± 912.9 | 29,336.5 ± 931.3 | 31,159.0 ± 1,032.4 | |
| 4 | 20,045.5 ± 1,076.9 | 27,693.0 ± 557.2 | 29,904.0 ± 421.4 | 30,953.0 ± 1,636.2 | ||
| 5 | 20,213.0 ± 616.6 | 28,993.0 ± 1,598.1 | 30,942.0 ± 2,330.6 | 32,734.0 ± 3,060.4 | ||
| 2.0 | 3 | 19,701.0 ± 130.1 | 26,147.0 ± 1,937.5 | 28,051.0 ± 1,876.7 | 28,416.5 ± 1,933.9 | |
| 4 | 20,869.0 ± 107.5 | 26,976.5 ± 163.3 | 27,241.5 ± 208.6 | 25,996.5 ± 200.1 | ||
| 5 | 19,501.0 ± 195.2 | 18,357.5 ± 198.7 | 16,745.5 ± 422.1 | 15,561.0 ± 246.1 | ||
| 20 | 1.0 | 3 | 20,302.0 ± 285.7 | 24,093.0 ± 567.1 | 32,072.0 ± 25.5 | 34,285.0 ± 397.4 |
| 4 | 20,089.0 ± 773.6 | 23,090.5 ± 5,574.1 | 28,874.5 ± 3,933.6 | 31,645.0 ± 4,881.9 | ||
| 5 | 20,318.0 ± 407.3 | 21,904.5 ± 1,109.5 | 23,833.0 ± 1,267.1 | 25,187.5 ± 2,813.6 | ||
| 1.5 | 3 | 19,722.0 ± 582.7 | 23,413.5 ± 1,502.6 | 27,653.0 ± 1,661.7 | 28,745.5 ± 2,911.2 | |
| 4 | 19,711.5 ± 37.5 | 21,665.0 ± 833.0 | 24,403.5 ± 1,017.5 | 24,942.5 ± 1,214.1 | ||
| 5 | 19,812.5 ± 502.8 | 21,306.0 ± 4,435.0 | 23,478.5 ± 4,995.7 | 24,689.0 ± 4,624.5 | ||
| 2.0 | 3 | 20,014.5 ± 178.9 | 21,600.0 ± 199.4 | 23,230.0 ± 418.6 | 23,930.0 ± 1,306.7 | |
| 4 | 19,838.5 ± 1,002.0 | 18,428.0 ± 3,477.6 | 19,365.0 ± 3,521.4 | 18,144.0 ± 3,572.3 | ||
| 5 | 20,006.5 ± 983.6 | 17,715.5 ± 2,468.5 | 16,451.0 ± 2,012.4 | 15,901.5 ± 1,777.0 | ||
| 25 | 1.0 | 3 | 20,675.0 ± 422.9 | 24,974.0 ± 86.3 | 25,920.5 ± 1,229.7 | 28,263.0 ± 502.0 |
| 4 | 20,793.0 ± 137.2 | 22,432.0 ± 899.4 | 27,501.0 ± 1,909.2 | 29,422.5 ± 176.1 | ||
| 5 | 20,123.5 ± 559.3 | 18,730.5 ± 1,685.0 | 18,160.0 ± 1,096.0 | 18,816.0 ± 1,803.1 | ||
| 1.5 | 3 | 19,862.5 ± 881.8 | 19,748.5 ± 729.0 | 20,576.0 ± 425.7 | 21,118.5 ± 484.4 | |
| 4 | 20,640.5 ± 218.5 | 20,664.5 ± 2,566.1 | 20,224.0 ± 3,289.5 | 21,041.5 ± 3,988.8 | ||
| 5 | 19,841.0 ± 181.0 | 18,418.0 ± 2,057.7 | 18,067.0 ± 2,005.4 | 17,752.0 ± 1,709.8 | ||
| 2.0 | 3 | 19,892.0 ± 427.1 | 19,822.0 ± 1,031.0 | 19,338.5 ± 685.2 | 18,049.0 ± 135.8 | |
| 4 | 20,040.0 ± 401.6 | 18,962.0 ± 3,094.3 | 17,608.5 ± 2,341.2 | 16,908.5 ± 2,868.7 | ||
| 5 | 19,424.5 ± 290.6 | 16,855.0 ± 763.7 | 13,936.5 ± 676.7 | 12,903.5 ± 441.9 | ||
| 30 | 1.0 | 3 | 19,361.0 ± 376.2 | 14,075.5 ± 1,446.0 | 15,328.0 ± 199.4 | 15,636.5 ± 57.3 |
| 4 | 19,722.5 ± 13.4 | 17,011.5 ± 324.6 | 16,768.5 ± 703.6 | 18,110.5 ± 1,727.5 | ||
| 5 | 20,218.0 ± 899.4 | 15,474.5 ± 3,428.8 | 14,213.0 ± 3,539.8 | 15,036.0 ± 4,061.6 | ||
| 1.5 | 3 | 20,756.5 ± 115.3 | 17,812.5 ± 1,949.5 | 18,356.0 ± 2,910.5 | 19,435.0 ± 3,863.6 | |
| 4 | 20,295.0 ± 646.3 | 16,943.5 ± 1,348.5 | 16,370.5 ± 1,778.4 | 17,551.0 ± 2,045.0 | ||
| 5 | 20,140.5 ± 444.8 | 16,376.5 ± 450.4 | 14,688.0 ± 445.5 | 15,563.0 ± 961.7 | ||
| 2.0 | 3 | 20,648.5 ± 432.0 | 15,291.5 ± 200.1 | 13,947.5 ± 217.1 | 14,818.5 ± 297.7 | |
| 4 | 20,292.0 ± 520.4 | 14,425.0 ± 2,259.9 | 13,129.0 ± 2,098.7 | 12,491.5 ± 1,880.2 | ||
| 5 | 19,352.5 ± 422.1 | 14,271.5 ± 840.8 | 11,024.0 ± 622.3 | 10,654.0 ± 352.1 |
Day1 represents the cell numbers per well of a 12-well plate, which were counted manually just after the seeding of cells. Day3 means the corresponding data read by a cell counter on the 3rd day, and so on. Differences were considered significant when P < 0.05
* P < 0.01 among different dose groups
# P < 0.01 among various dose rate groups
& P < 0.05 among different combinations of dose and dose rate
^ P < 0.01 between group P3–P4 and group P5