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. 2014 Dec 31;68(4):839–848. doi: 10.1007/s10616-014-9837-6

Table 1.

Primer sequences for real-time PCR

Gene Primer sequence (5′–3′) Product size Accession number
Klf2 F: AAGACCTACACCAAGAGTTCGCATC
R: TCTGAGCGCGCAAACTTCC
110 NM_016270.2
Klf5 F: ACGCATCCACTACTGCGATTACC
R: AACCTCCAGTCGCAGCCTTC
132 NM_001730.3
Klf4 F: AAGAGTTCCCATCTCAAGGCACA
R:GGGCGAATTTCCATCCACAG
91 NM_004235.4
Oct4 F: CTTGCTGCAGAAGTGGGTGGAGGAA
R: CTGCAGTGTGGGTTTCGGGCA
169 NM_001159542.1
Nanog F: GAACTCTCCAACATCCTGAACCTC
R: CCTTCTGCGTCACACCATTGC
127 NM_024865.2
Rex1 F: GGAATGTGGGAAAGCGTTCGT
R: CCGTGTGGATGCGCACGT
152 NM_174900.3
ON F: CGAGCTGGATGAGAACAACA
R: AAGTGGCAGGAAGAGTCGAA
126 BC072457
COMP F: AGGGAGATCGTGCAGACAA
R: AGCTGGAGCTGTCCTGGTAG
154 NM_000095
LPL F: AGAGCCAAAAGAAGCAG
R: GGCAGAGTGAATGGGAT
182 NM_000237
β-actin F: CACACTGTGCCCATCTACGA
R: TGAGGATCTTCATGAGGTAGTCAG
106 GZ549052