Fig. 6.

Xenoantigenic Neu5Gc contamination and expression of HLA-DR on MSCs. BM-MSCs cultured in a FBS-containing medium stained positively with anti-Neu5Gc whereas cells cultured in b PL1- and in c PL2-media did not have Neu5Gc on their surface. HLA-DR was not expressed on BM-MSCs cultured in d FBS-containing medium but was found on cells cultured in e PL1- and f PL2-medium. Filled histograms represent the unspecific/isotype controls and the solid line the specific staining