Table 1.

Summary of results obtained with the Drosophila wing SMART in the marker-heterozygous and balancer-heterozygous wings after treatment with bupivacaine: Results with the mwh/flr ³ and mwh/TM3 genotypes

Test concentration (µg/mL)	Number of wings (N)	Small single spots (1–2 cells) (m = 2)			Large single spots (>2 cells) (m = 5)			Twin spots (m = 5)			Total mwh spots (m = 2)			Total spots (m = 2)			Frequency of clone formation per 105 cells
		No	Fr.	D	No	Fr.	D	No	Fr.	D	No	Fr.	D	No	Fr.	D
(A) Standard cross (ST)
Marker heterozygous wings (mwh/flr 3)
1 % DMSO	50	2	0.04		0	0.00		0	0.00		2	0.04		2	0.04		0.16
10	40	5	0.13	i	0	0.00	i	0	0.00	i	5	0.13	i	5	0.13	i	0.51
100	40	5	0.13	i	0	0.00	i	0	0.00	i	5	0.13	i	5	0.13	i	0.51
250	40	6	0.15	i	0	0.00	i	0	0.00	i	6	0.15	i	6	0.15	i	0.62
500	40	4	0.10	i	2	0.05	i	0	0.00	i	6	0.15	i	6	0.15	i	0.62
(B) High-bioactivation cross (HB)
Marker heterozygous wings (mwh/flr 3)
1 % DMSO	40	6	0.15		0	0.00		0	0.00		6	0.15		6	0.15		0.62
10	40	4	0.10	i	2	0.05	i	0	0.00	i	6	0.15	i	6	0.15	i	0.62
100	40	3	0.08	–	2	0.05	i	0	0.00	i	5	0.13	i	5	0.13	i	0.51
250	40	2	0.05	–	1	0.03	i	0	0.00	i	3	0.08	–	3	0.08	–	0.31
500	40	1	0.03	–	0	0.00	i	0	0.00	i	1	0.03	–	1	0.03	–	0.10

No number, Fr frequency, D statistical diagnosis according to Frei and Würgler (1988), + positive, − negative, i inconclusive, m multiplication factor, probability levels a = b = 0.05