Table 2.

Summary of results obtained with the Drosophila wing SMART in the marker-heterozygous and balancer-heterozygous wings after treatment with levobupivacaine: results with the mwh/flr ³ and mwh/TM3 genotypes

Test concentration (µg/mL)	Number of wings (N)	Small single spots (1–2 cells) (m = 2)			Large single spots (>2 cells) (m = 5)			Twin spots (m = 5)			Total mwh spots (m = 2)			Total spots (m = 2)			Frequency of clone formation per 105 cells
		No	Fr.	D	No	Fr.	D	No	Fr.	D	No	Fr.	D	No	Fr.	D
(A ) Standard cross (ST)
Marker heterozygous wings (mwh/flr 3)
Distilled water	90	9	0.10		0	0.00		0	0.00		9	0.10		9	0.10		0.41
100	40	4	0.10	i	0	0.00	i	0	0.00	i	4	0.10	i	4	0.10	i	0.41
250	40	6	0.15	i	1	0.03	i	2	0.05	i	9	0.23	i	9	0.23	i	0.92
500	40	3	0.08	i	1	0.03	i	0	0.00	i	4	0.10	i	4	0.10	i	0.41
1000	40	4	0.10	i	2	0.05	i	0	0.00	i	6	0.15	i	6	0.15	i	0.62
(B) High-bioactivation cross (HB)
Marker heterozygous wings (mwh/flr 3)
Distilled water	40	5	0.13		1	0.03		0	0.00		6	0.15		6	0.15		0.62
100	40	14	0.35	+	0	0.00	i	0	0.00	i	14	0.35	i	14	0.35	i	1.43
250	40	8	0.20	i	2	0.05	i	0	0.00	i	10	0.25	i	10	0.25	i	1.03
500	40	10	0.25	i	0	0.00	i	0	0.00	i	10	0.25	i	10	0.25	i	1.03
1000	40	14	0.35	+	3	0.08	i	0	0.00	i	17	0.43	+	17	0.43	+	1.74
Balancer heterozygous wings (mwh/TM3)
Distilled water	40	5	0.13		0	0.00					5	0.08		5	0.08		0.51
100	40	4	0.10	i	0	0.00	i				4	0.10	i	4	0.10	i	0.41
250	40	2	0.05	–	1	0.03	i				3	0.08	i	3	0.08	i	0.31
500	40	0	0.00	–	0	0.00	i				0	0.00	–	0	0.00	–	0.00
1000	40	0	0.00	–	0	0.00	i				0	0.00	–	0	0.00	–	0.00

No number, Fr frequency, D statistical diagnosis according to Frei and Würgler (1988), + positive, − negative, i inconclusive, m: multiplication factor, probability levels a = b = 0.05