Fig. 2.

Molecular and cellular characterization of HEKs: a morphology of HEKs shows cohesive polygonal cells filled with granular cytoplasm. b H&E staining showing polygonal architecture of primary keratinocytes. c RT–PCR studies revealed the expression key HEK markers. Immunocytochemistry of HEKs demonstrated uniform fluorescence when immunostained against d–f E-cadherin, g–i Keratn-5, j–l Keratin-8, and m–o cytokeratin-7 using specific antibodies. 4,6-diamino-2-phenylindole (DAPI) was used for each sample as a nuclear stain. Green colour represents FITC conjugate. Scale bars 100 μm (a, b, j–o), 200 μm (g–i), 400 μm (d–f). (Color figure online)