Table 2.

Represents the list of genes primers along with the forward and reverse sequences, Annealing conditions and region of amplification

Gene symbol	Primer sequences (5′-3′)	Tm	Size
β-actin	5′-TCACCCACACTGTGCCCATCTACGA-3′ 5′-CAGCGGAACCGCTCATTGCCAATGG-3′	58 °C	294
Keratin-19	5′-TGAGGTCATGGCCGAGCAGAAC-3′ 5′-CATGAGCCGCTGGTACTCCTGA-3′	58 °C	331
Keratin-8	5′-CAGATCAAGTATGAGGAGCTGCA-3′ 5′-AGCTGGTGCGGCTGAAGGAT-3′	58 °C	329
Keratin-18	5′-CCATGCGCCAGTCTGTGGAG-3′ 5′-GTGGTGCTCTCCTCA ATCTGCT-3′	58 °C	322