Table 3.
Represents the list of antibodies used to characterize the HSKs and DMSCs
| Antigen | Antibody | Dilution used | Brand and cat. no. | Application |
|---|---|---|---|---|
| E-cadherin | E-Cadherin, mouse IgG2a | 1:50 | BD transduction laboratories (610181) | Cultured cells (in situ staining) and flow cytometry |
| Keratin-5 | Anti-cytokeratin 5 antibody (Keratin-5) | 1:50 | Millipore (Temecula, CA, USA) (MAB1620) | Immunohistochemistry, immunofluorescence and flow cytometry |
| Keratin-8 | Anti-keratin epithelial antibody (Keratin-8) | 1:50 | Millipore (MAB1611) | Immunohistochemistry and western blotting |
| Cytokeratin-7 | Cytokeratin-7, mouse IgG1 | 1:100 | BD Pharmingen (550507) | Immunohistochemistry and western blotting |
| Vimentin | Monoclonal mouse immunoglobulin | 1:100 | Millipore (MAB3400) | Cultured cells (in situ staining) and flow cytometry |
| CD-44 | Anti-human CD44 | 1:50 | BD Pharmingen (550989) | Immunophenotyping by flow cytometry |
| CD-73 | Anti-human CD73 | 1:50 | BD Pharmingen (550257) | Immunophenotyping by flow cytometry |
| CD-90 | Anti-human CD90 | 1:50 | BD Pharmingen (555596) | Immunophenotyping by flow cytometry |
| CD-166 | Anti-human CD166 | 1:50 | BD Pharmingen (559263) | Immunophenotyping by flow cytometry |
| HLA-DR | Anti-human HLA-DR | 1:50 | BD Pharmingen (347363) | Immunophenotyping by flow cytometry |
| CD-45 | Anti-human CD45 | 1:50 | BD Pharmingen (555482) | Immunophenotyping by flow cytometry |
| CD-34 | Anti-human CD34 | 1:50 | BD Pharmingen (555821) | Immunophenotyping by flow cytometry |