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. 2015 Mar 6;68(4):1063–1080. doi: 10.1007/s10616-015-9862-0

Fig. 4.

Fig. 4

a The results of real-time PCR assay using PromoKine’s PCR Mycoplasma Test Kit I/RT performed with specific primers and probes (Promokine diagnostic kit) on different serial dilutions of DNA obtained from a culture of M. hyorhinis Amplification curve 1 negative control (DNA-free water), 2 Undiluted-DNA strain of M. hyorhinis (30 ng, positive), 3 3 ng (positive), 4 300 pg (positive), 5 30 pg (positive), 6 3 pg (positive), 7 300 fg (negative), 8 30 fg (negative), 9 3 fg (negative), 10 positive control kit. b Gel electrophoresis results of the real-time PCR assay products. Lane 1 DNA Size marker (100 bp DNA Ladder, Roche XIV), Lane 2 negative control (DNA-free water), lane 3 Undiluted-DNA strain of M. hyorhinis (30 ng, positive), lane 4 3 ng (positive), lane 5 300 pg (positive), lane 6 30 pg (positive), lane 7 3 pg (positive), lane 8 300 fg (negative), lane 9 30 fg (negative), lane 10 3 fg (negative), lane 11. The kit positive control. FAM Fluorescein amidite (6-carboxyfluoresceine), VIC a proprietary fluorescent dye produced by Applied Biosystems. Detection of target amplification was performed in FAM channel whereas monitoring of inhibition control amplification in VIC channel.