Table 3.
The evaluation results of 30 cell lines for the detection of mycoplasma contamination by five different methods (microbial culture, DAPI staining, enzymatic mycoalert® ,method, molecular PCR and real-time PCR assays)
| No. | NCBI code | Cell lines | Test results | ||||
|---|---|---|---|---|---|---|---|
| Microbial culture | Mycoalert® | DAPI staining | PCR | Real time PCR | |||
| 1 | NCBI C635 | HEK-Blue-Null-1 | Negative | Negative | Negative | Negative | Negative |
| 2 | NCBI C579 | P3U1 | Negative | Negative | Positive (false-positive) | Negative | Negative |
| 3 | NCBI C606 | NB2-11 | Negative | Negative | Positive (false-positive) | Negative | Negative |
| 4 | NCBI C623 | Densovirus free C6/36 Aedes albopictus | Negative | Negative | Negative | Negative | Negative |
| 5 | NCBI C608 | BFA | Positive | Positive | Positive | Positive | Positive |
| 6 | NCBI C639 | RAW 264.7 | Negative (false-negative) | Positive | Positive | Positive | Positive |
| 7 | NCBI C638 | SW872 | Positive | Positive | Positive | Positive | Positive |
| 8 | NCBI C624 | NCCIT | Positive | Positive | Positive | Positive | Positive |
| 9 | NCBI C625 | D-17 | Negative (false-negative) | Positive | Positive | Positive | Positive |
| 10 | NCBI C615 | MKN-45 | Positive | Positive | Positive | Positive | Positive |
| 11 | NCBI C570 | HCT-116 | Negative | Negative | Negative | Negative | Negative |
| 12 | NCBI C609 | MCF10A | Negative | Negative | Negative | Negative | Negative |
| 13 | NCBI C627 | CF41.Mg | Negative (false-negative) | Positive (weak positive) | Negative (false-negative) | Negative (false-negative) | Positive |
| 14 | NCBI C532 | CT-26 | Negative (false-negative) | Positive (weak positive) | Positive | Positive | Positive |
| 15 | NCBI C566 | SKLC6 | Negative | Negative | Negative | Negative | Negative |
| 16 | NCBI C622 | BHY | Positive | Positive | Positive | Positive | Positive |
| 17 | NCBI C595 | Caov-4 | Positive | Positive | Negative (false-negative) | Positive | Positive |
| 18 | NCBI C537 | STO | Negative (false-negative) | Positive | Negative (false-negative) | Positive | Positive |
| 19 | NCBI C511 | PEER | Positive | Negative (false-negative) | positive | Positive | Positive |
| 20 | NCBI C610 | GH3/B6 | Positive | positive | positive | Positive | Positive |
| 21 | NCBI C593 | F25 | Negative | Negative | Positive (false-positive) | Negative | Negative |
| 22 | NCBI C590 | CHO/dhfr− | Negative | Negative | Negative | Negative | Negative |
| 23 | NCBI C552 | YAC1 | Negative | Negative | Negative | Negative | Negative |
| 24 | NCBI C523 | RK13 | Negative | Negative | Negative | Negative | Negative |
| 25 | NCBI C602 | TF-1 | Negative | Negative | Negative | Negative | Negative |
| 26 | NCBI C611 | SH-SY5Y | Negative (false-negative) | Negative (false-negative) | Negative (false-negative) | Positive | Positive |
| 27 | NCBI C614 | BE(2)-M17 | Negative (false-negative) | Positive | Negative (false-negative) | Positive | Positive |
| 28 | NCBI C617 | OLN-93 | Negative (false-negative) | Positive | Negative (false-negative) | Positive | Positive |
| 29 | NCBI C628 | MCF-HGH | Positive | Positive | positive | Positive | Positive |
| 30 | NCBI C620 | C28/I2 | Positive | Positive | Negative (false-negative) | Positive | Positive |