Fig. 10.

The influence of the MEM-H(0) age on the viability of MRC-5 cells incubated according to the rubella virus harvesting regime. Cell cultures were incubated in either fresh (empty columns) or 9-month old (stored) (dashed columns) medium and analysed by MTT assay (A), and by flow cytometric determination of necrotic (B) and apoptotic (C) cells percentage. Standard deviations are shown (n = 4 in A; n = 3 in B and C), asterisk indicates the statistically significant differences between the two media (t statistic: p < 0.05)