Fig. 8.

The influence of the MEM-H(0) age on the viability of CEF. Cell cultures were incubated in either fresh (empty columns) or 9-month old (stored) (dashed columns) medium according to the mumps virus harvesting regime and analysed by MTT assay (A), and by flow cytometric determination of necrotic (B) and apoptotic (C) cells percentage. Standard deviations are shown (n = 4 in A; n = 3 in B and C), asterisk indicates the statistically significant differences between the two media (t statistic: p < 0.05)