Table 2.

The effect of the MEM-H(N)-FBS medium age on the cell growth

Test points (months)	Batch	Cell culture
		p (significant if <0.05)
		Vero	MRC-5	WI-38
3	1	0.81732	0.30963	0.00039
	2	0.68604	0.23323	0.75704
	3	0.86237	0.01517*	0.00127
6	1	0.95395	0.23299	n.t.
	2	0.68611	0.19996	n.t.
	3	0.97696	0.30988	n.t.
9	1	0.70739	0.00708	n.t.
	2	0.40230	0.45291	n.t.
	3	0.56353	0.69110	n.t.
12	1	0.14892	n.t.	n.t.
	2	0.81721	0.12228	n.t.
	3	0.88521	n.t.	n.t.

Growth curves (log phase) of the cells cultivated in the aged media were compared to the ones cultivated in the fresh batch to assess whether the cell culture capability to grow was affected

Significant values (<0.05) are given in bold

n.t. not tested

* Statistically significant, but in favor of the aged medium