Fig. 2.

USP-13-Med cells are capable of generating tumorspheres at high efficiency. a Total number of tumorspheres over 50 µm in diameter generated from USP-13-Med and Daoy cells, after 7 days of culture under low-attachment conditions. Representative images of tumorspheres generated by Daoy (b) and USP-13-Med (c) cells are shown. Bar size 400 µm. d, e Population of CD133⁺ cells in USP-13-Med and Daoy tumorspheres compared with standard culture condition (adherent). The flow cytometry analysis shows an enrichment in CD133⁺ cells in USP-13-Med tumorspheres. Dashed line Negative Control; Gray Experimental condition. ***p < 0.001