Fig. 4.

Migration and invasion capabilities of USP-13-Med cells. a Representative images from the wound healing-like migration assay with USP-13-Med and Daoy cells after 0, 12 and 24 h of assay. Bar size 500 µm. b Quantification of migrated area after 12 and 24 h. c Representative images of USP-13-Med and Daoy tumor spheroids embedded in invasion matrix at days 0, 2 and 4. The spindle-like protrusions observed are formed by tumor cells invading the matrix. The higher the cell invasion, the higher the length and amount of such protrusions. d Measurement of area invaded by USP-13-Med and Daoy cells after 2 and 4 days of assay. Bar size 500 µm. *p < 0.05