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. 2016 Jul 26;10:180. doi: 10.3389/fncel.2016.00180

FIGURE 1.

FIGURE 1

Expression and localization of CAP2 in brain. (A) Western blot analysis of CAP2 in lysates from dissected brain regions at different developmental stage. (B) Western blot analysis of CAP1 in lysates from dissected brain regions at different developmental stage. (C) Immunocytochemistry of cultured cortical pyramidal neurons demonstrates the presence of CAP2 (green) in the dendritic shaft and mature spine. F-actin rich structures were visualized with TRITC-phalloidin (red). Scale bar, 10 μm. (D) Co-labeling with antibodies against VGLUT1 (blue) revealed the presence of CAP2 in excitatory presynaptic terminal of pEGFP-C2 labeled neurons (merge, white asteriks). However, homogeneous distribution was observed in the axonal shaft. Scale bar, 10 μm. (E) Co-labeling of cultured cortical neurons with antibodies against CAP2 (red) and PSD-95 (blue) revealed that CAP2 localizes with PSD-95 (white dashed square). Scale bar, 5 μm.