Skip to main content
. 2016 Jul 26;10:180. doi: 10.3389/fncel.2016.00180

FIGURE 4.

FIGURE 4

(A) pEGFP-C2 transfected primary cortical neurons at div 16 labeled with antibodies against vGLUT1 (a presynaptic marker, red) and PSD-95 (a postsynaptic marker, blue). An increase in excitatory synapse (white asterik) was observed in CAP2 mutant neurons (WT: 3.1 ± 1.1 synapses/10 μm, n = 20 neurons from 4 experiments; Cap2gt/gt: 7.1 ± 1.0 synapses/10 μm, n = 20 neurons from 3 experiments, p < 0.01). Scale bar, 5 μm. (B) pEGFP-C2 transfected primary cortical neurons at div 16 labeled with antibodies against VGAT (an inhibitory presynaptic marker, red) and gephyrin (an inhibitory postsynaptic marker, blue). There is no significant difference in inhibitory synapses (white asterisk) in CAP2 mutant neurons (WT: 4.5 ± 1.0 synapses/10 μm, n = 20 neurons from 4 experiments; Cap2gt/gt: 4.1 ± 1.1 synapses/10 μm, n = 20 neurons from 3 experiments, p > 0.05). Scale bar, 5 μm. **p < 0.01.