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. 2016 Jul 26;10:52. doi: 10.3389/fncir.2016.00052

FIGURE 1.

FIGURE 1

Efficacy of Arch expression in SOM cells. (A) Fluorescent image of Arch-EYFP-expressing SOM cells throughout barrel cortex; scale bar = 200 μm. Inset: expanded view of 2 Arch-expressing SOM cells, scale bar = 20 μm. (B) Light-evoked hyperpolarization (500 ms pulse duration) of Arch-expressing SOM cells in layer 5 as 40x immersion objective is moved away from the recorded cell in 70 μm increments (n = 6 cells). Values plotted on green curve are peak values of hyperpolarization and values plotted on blue curve are steady-state values of hyperpolarization (measured during 50-ms period before offset of light stimulus). (C) As in (B), but for Arch-expressing SOM cells in layer 2/3 (n = 6 cells). (D) Light stimuli significantly decrease spiking output of layer 2/3 Arch-expressing SOM cells during evoked Up states. Example voltage trace is shown above. Shown below are population data (n = 8 cells; Dark: 11.1 ± 2.8 Hz, LED: 3.6 ± 1.7 Hz, P = 0.014, paired t-test). (E) Light stimuli completely silence Up state spiking in all recorded layer 5 Arch-expressing SOM cells. Example voltage trace is shown above. Shown below are population data (n = 6 cells; Dark: 4.7 ± 1.1 Hz, LED: 0 Hz, P = 0.007, paired t-test). Action potentials are truncated in (D,E).