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. 2016 Jul 26;7:1157. doi: 10.3389/fmicb.2016.01157

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Copyright © 2016 Ottman, Huuskonen, Reunanen, Boeren, Klievink, Smidt, Belzer and de Vos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sarkosyl extraction leads to enrichment of OM and intracellular proteins. Subcellular localization of proteins identified from the OM and intracellular fraction of A. muciniphila grown on mucin (A) or glucose (B). CELLO was used to predict protein localization. Relative abundances of the proteins are presented on a log10 scale with the dashed diagonal line indicating an even distribution between OM and intracellular fraction. A relative abundance of 4.0 represents proteins that were not detected or were under the detection limit. All proteins with LFQ intensity above 8.5 in the sarkosyl-extracted OM fraction (horizontal dashed line) were included in the preliminary list of potential OM proteins.