FIGURE 4.

Expression analysis of flagella and type IV pili-related genes in Lysobacter capsici AZ78. The expression of genes encoding flagellar (A) and type IV pili (B) regulatory and structural proteins was analyzed when L. capsici AZ78 was grown for 20 h on Luria Bertani Agar (LBA 0.5), Swarming Agar (SWR) and Pea Agar Medium (PAM 0.5); the media were amended with 0.5% Agar (w/v). Relative expression levels were calculated using recA as the constitutive gene for data normalization, and data were calibrated on L. capsici AZ78 growth on LBA 0.5 samples. An F-test revealed non-significant differences between experiments (P values ranged from 0.08 to 0.96, for the genes tested), and data from the two experiments were pooled. Mean and standard error values were calculated as the pool of six replicates from two experiments. For each gene, different letters indicate significant differences according to Tukey’s test (α = 0.05). (A) Flagellar genes: 1, flhB; 2, fliR; 3, flgE; 4, flgI; 5, fliH; 6, fliA. (B) T4P genes: 1, pilA.1; 2, pilA.2; 3, pilZ; 4, pilY1; 5, pilB; 6, clp; 7, fimX; 8, rpoN; 9, pilM; 10 pilG; 11, pilI; 12, pilL; 13, pilJ; 14, pilQ.