Table 1.
Kinetic parameters and optimal conditions for TR activity of the indicated E. coli flavoenzymes.
| Enzyme | TR activity (U/mg protein) | Km apparent (mM) | Ki apparent (mM) | Vmax (U/mg protein) | pH | Temp (°C) | Cofactor |
|---|---|---|---|---|---|---|---|
| GorA | 32,228.6 ± 588.8 | 0.08947 | 0.2327 | 6,314 | 9 | 37 | NADPH |
| E3 | 2,788.1 ± 148.9 | 0.04794 | 0.06301 | 84.5 | 6 | 37 | NADPH |
| TrxB | 1,423.9 ± 181.7 | 0.1145 | 0.03713 | 9,586 | 10 | 37 | NADPH |
| AhpF | 931.1 ± 17.6 | 0.8196 | – | 77,875 | 10 | 37 | NADH |
| YkgC | 874.7 ± 41.8 | 0.5171 | – | 2,696 | 9 | 37 | NADH |
| NorW | 664.1 ± 231.1 | 0.6949 | – | 5,347 | 11 | 42 | NADH |
TR activity and kinetic parameters were determined as described in Section “Materials and Methods.