Table 8.
Out of the 190 small secreted cysteine-rich proteins (SSCPs) identified in the genome of F. graminearum (isolate PH-1) by Lu and Edwards (2016), we could assign 48 exclusively to active plant (AP), passive plant (PP), or DON- inducing (DI) up- or down-regulated categories within our analysis (indicated by gray colored fields).
| FGSG number | Description | AP | PP | DI | |||
|---|---|---|---|---|---|---|---|
| Up | Down | Up | Down | Up | Down | ||
| FGSG_00056 | Uncharacterized protein | ||||||
| FGSG_00060** | Related to KP4 killer toxin | ||||||
| FGSG_00061** | Related to KP4 killer toxin | ||||||
| FGSG_00062** | Related to KP4 killer toxin | ||||||
| FGSG_00114 | Uncharacterized protein | ||||||
| FGSG_00230 | Uncharacterized protein | ||||||
| FGSG_01239 | Uncharacterized protein | ||||||
| FGSG_01771 | Uncharacterized protein | ||||||
| FGSG_01831** | Related to trihydrophobin precursor | ||||||
| FGSG_02685 | Uncharacterized protein | ||||||
| FGSG_02935 | Uncharacterized protein | ||||||
| FGSG_03130 | Uncharacterized protein | ||||||
| FGSG_03157 | Uncharacterized protein | ||||||
| FGSG_03326 | Uncharacterized protein | ||||||
| FGSG_03599** | Uncharacterized protein | ||||||
| FGSG_03894 | Uncharacterized protein | ||||||
| FGSG_03911 | Uncharacterized protein | ||||||
| FGSG_03960 | Uncharacterized protein | ||||||
| FGSG_04661** | Uncharacterized protein | ||||||
| FGSG_04745 | Related to antifungal protein | ||||||
| FGSG_04805** | Uncharacterized protein | ||||||
| FGSG_05714* | Uncharacterized protein | ||||||
| FGSG_07807 | Uncharacterized protein | ||||||
| FGSG_07972 | Uncharacterized protein | ||||||
| FGSG_08085 | Uncharacterized protein | ||||||
| FGSG_08122* | Uncharacterized protein—related to cell wall protein phia | ||||||
| FGSG_08142 | Uncharacterized protein | ||||||
| FGSG_08164* | Uncharacterized protein | ||||||
| FGSG_09066 | Uncharacterized protein | ||||||
| FGSG_09133 | Uncharacterized protein | ||||||
| FGSG_09403 | Uncharacterized protein | ||||||
| FGSG_10554 | Uncharacterized protein | ||||||
| FGSG_11047 | Uncharacterized protein | ||||||
| FGSG_11205* | Probable snodprot1 precursor | ||||||
| FGSG_11225 | Uncharacterized protein | ||||||
| FGSG_11540 | Uncharacterized protein | ||||||
| FGSG_11564 | Uncharacterized protein | ||||||
| FGSG_12554 | Uncharacterized protein | ||||||
| FGSG_12673 | Uncharacterized protein | ||||||
| FGSG_12722 | Uncharacterized protein | ||||||
| FGSG_12981 | Uncharacterized protein | ||||||
| FGSG_13412 | Uncharacterized protein | ||||||
| FGSG_13462 | Uncharacterized protein | ||||||
| FGSG_13464 | Uncharacterized protein | ||||||
| FGSG_13491 | Uncharacterized protein | ||||||
| FGSG_13505 | Uncharacterized protein | ||||||
| FGSG_13952** | Uncharacterized protein | ||||||
| FGSG_14010 | Uncharacterized protein | ||||||
Of these, 12 genes are among the 34 SSCPs detected by Lu and Edwards (2016) during transcriptional analysis of infected wheat heads- FGSG numbers marked by one asterisk (*). FGSG numbers marked by two asterisks (**) are among the 15 in planta SSCPs with transcripts up-regulated at certain point. Underlined FGSG numbers are among the 25 SSCPs confirmed by nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) analysis of the minimal medium-based in vitro secretome.