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. 2016 Jul 26;7:1150. doi: 10.3389/fmicb.2016.01150

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Copyright © 2016 Merino and Tomás.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purified HBBs of polar and lateral flagella. (A) 12 and 7.5% SDS-PAGE of purified flagella HBB. The black arrows show FlaA and FlaB polar flagellins. The asterisk shows a band which molecular weight correlate to MotY and MotX proteins. (B) Western blot analysis using A. hydrophila AH-3 FlgT antiserum (1:1,000). Size standard (St); polar flagella HBB of AH-3 (1), AH-3ΔflgT mutant (2); purified His6-FlgT protein (3); lateral flagella HBB of AH-3::flhA (4); and AH-3ΔflgT mutant complemented with pBAD33-FLGT grown under inducer condition (5).