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. 2016 Jul 26;7:1150. doi: 10.3389/fmicb.2016.01150

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Copyright © 2016 Merino and Tomás.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Adhesion of A. hydrophila AH-3, non-polar flagellum mutant AH-3::flaAΔflaB, AH-3ΔflgT mutant, and AH-3ΔflgT complemented with pBAD33-FLGT to HEp-2 cells. Mean number of adherent bacteria per HEp-2 cells. (B) Biofilm formation ability of A. hydrophila AH-3, non-polar flagellum mutant AH-3::flaAΔflaB, AH-3ΔflgT mutant, and AH-3ΔflgT complemented with pBAD33-FLGT. OD₅₇₀ quantifies the amount of crystal violet retained by the biofilm on the microtiter plates after staining. The complemented mutant was grown under induced conditions (0.2% L-arabinose). The average of three independent experiments (each experiment performed in duplicate) is show. Error bars represent standard deviations.