Skip to main content
. 2016 Jul 26;7:227. doi: 10.3389/fphar.2016.00227

FIGURE 2.

FIGURE 2

Scorpion venom heat-resistant peptide reduced β-amyloid oligomers in C. elegans. (A) Representative western blot of Aβ species in CL2006, CL4176, and CL802 worms fed vehicle or SVHRP. CL2006 maintained at 20°C fed with a vehicle (Ctrl), SVHRP (40 μg/ml) for 96 h. CL4176 and CL802 worms fed with a vehicle (Ctrl), SVHRP (40 μg/ml) were maintained for 36 h at 16°C, then the temperature was raised from 16 to 23°C. 25 h later, the worms were collected and equal amounts of protein were loaded in each lane and immunoblotted with anti-Aβ antibody (6E10) or tubulin. Arrows indicate the Aβ oligomers (20 kDa). Quantification of Aβ oligomers (the band at 20 kDa) in CL2006 (B) and CL4176 (C) worms fed either vehicle or SVHRP using ImageJ software. Data are expressed as mean density of the indicated band based from three independent experiments. Error bars represent SD. ∗p < 0.05.