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Immunoblot analysis of selected PSRPs. An aliquot of 50 μg soluble proteins was separated by 12.5% SDS–PAGE, blotted onto Hybond-C membrane, processed for one-dimensional immunoblot analysis and quantified by densitometry. Immunoreactive protein levels for DnaK and MSR was detected using the respective primary antibodies and signals were detected. U represents unstressed fruit. Commassie blue-stained gel indicates equal protein loading across time points.
