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Representative microphotographs of preproghrelin mRNA-expressing cells in the stomach of PBS-treated control rats (n = 10) (A) and MTX-treated rats (n = 7) (B) revealed by in situ hybridization with DIG-labeled preproghrelin anti-sense riboprobes. (C) Quantification of the number of preproghrelin mRNA-positive cells in the gastric mucosal and submucosal layers of control and MTX-treated rats. Student’s t-test, ***p < 0.001, KS test p > 0.1 both (mean ± SEM). Scale bar, 100 μm.
