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Plasma concentrations of free (A,D) and total (B,E) IgG reactive with ghrelin (A,B) and des-acyl ghrelin (D,E) in MTX-treated (n = 10) and PBS-treated control rats (n = 10). Mann–Whitney or Student’s t-test (A,E) according to the KS normality tests, *p < 0.05, **p < 0.01, ***p < 0.001. KS tests, (A) p > 0.1 and p = 0.08, (B) p > 0.1 and p = 0.03, (C) p > 0.1 and p = 0.003, (D) p > 0.1 and p = 0.005, (E) p > 0.1 and p = 0.1, (F) p > 0.1 and p = 0.006 (mean ± SEM).
