Table 1. Activity of Cry51Aa2 protein variants on L. hesperus and L. lineolaris (LC₅₀ values in μg protein per ml).

Protein	Amino-acid difference(s) relative to Cry51Aa2	L. hesperus				L. lineolaris
		LC50	95% CI	Slope±s.e.	Chi-square	LC50	95% CI	Slope±s.e.	Chi-square
Cry51Aa2	Parent	73	50.2–116.35	1.77±0.27	43.28	>223	Not estimated
Cry51Aa2.807_5	HYS_ Δ(196–201)	22.4	14.8–39.9	1.93±0.35	29.48	ND
Cry51Aa2.834	F46S, Y54H, S167R, S217N, HYS_ Δ(196–201)	5.9	3.95–9.35	1.52±0.21	52.86	>223	Not estimated
Cry51Aa2.834_2	F46S, Y54H, T93A, S167R, S217N, HYS_ Δ(196–201)	2.9	2.25–3.85	2.12±0.25	72.22	ND
Cry51Aa2.834_3	F46S, Y54H, S95A, S167R, S217N, HYS_ Δ(196–201)	2.4	1.15–5.8	1.94±0.47	17.16	ND
Cry51Aa2.834_4	F46S, Y54H, F147A, S167R, S217N, HYS_ Δ(196–201)	1.1	0.7–1.7	1.30±0.16	64.43	ND
Cry51Aa2.834_6	F46S, Y54H, T93A, F147A, S167R, S217N, HYS_ Δ(196–201)	1.45	1.0–2.15	2.06±0.30	46.28	ND
Cry51Aa2.834_7	F46S, Y54H, Q149E, S167R, S217N, HYS_ Δ(196–201)	1.4	0.87–2.34	1.77±0.29	37.54	ND
Cry51Aa2.834_5	F46S, Y54H, S95A, F147A, S167R, S217N, HYS_ Δ(196–201)	0.8	0.6–1.15	1.82±0.21	72.08	223	153.5–314	1.84±0.55	11.15
Cry51Aa2.834_13	F46S, Y54H, S95A, F147A, S167R, P219R, HYS_ Δ(196–201)	9.9	4.45–10.15	1.03±0.10	105.38	8.3	7.85–9.35	3.16±0.57	31.27
Cry51Aa2.834_14	F46S, Y54H, S95A, F147A, S167R, P219R, V251A, HYS_ Δ(196–201)	0.6	0.1–1.6	1.01±0.26	14.81	4.8	3.05–6.55	2.98±0.38	60.02
Cry51Aa2.834_15	F46S, Y54H, S95A, F147A, S167R, P219R, R273W, HYS_ Δ(196–201)	1.35	0.95–1.93	1.75±0.23	59.35	5.9	5.0–6.6	2.04±0.35	34.7
Cry51Aa2.834_16	F46S, Y54H, S95A, F147S, Q149E, S167R, P219R, R273W, HYS_ Δ(196–201)	0.3	0.05–0.75	0.94±0.26	12.86	0.85	0.5–1.5	1.07±0.15	48.65
Cry51Aa2.834_17	F46S, Y54H, S95A, F147A, S167R, P219R, N239A, V251A, HYS_ Δ(196–201)	0.4	0.15–0.8	0.94±0.14	44.43	1.2	0.85–1.7	0.95±0.10	82.24

CI, confidence interval; ND, not determined.

HYS_Δ196–201=contiguous HYS deletion in residue range 196–201, n=40 (replications=4–5).