Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 18;7:12224. doi: 10.1038/ncomms12224

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Far-western blot using His-Suv39h1 full-length (WT) or truncated versions as baits and purified recombinant GST-Ubc9 as prey. We revealed bound GST-Ubc9 using anti-GST antibodies. Along each construct, interaction (+) or absence of interaction (−) with Ubc9 is indicated. (b) In vitro HP1α sumoylation assay. Top: western blot analysis of the sumoylation reaction mixture with anti-HP1α antibodies revealed sumoylated GST-HP1α (SUMO-1-GST-HP1α, box) and unmodified GST-HP1α. We performed quantitative analysis of this assay. The histogram shows the comparison of the ratio between SUMO-1-GST-HP1α and unmodified HP1α signal from western blot in the presence of Suv39h1-WT or truncated versions (Supplementary Fig. 5c). Error bars represent s.d. from three independent experiments. Bottom: results summary.