Figure 3. L. major induces increased number of WASp KO CD8⁺IFNγ⁺ T cells.

(a) Ear infiltration of cells. (a) Ears from WT and WASp KO control or L. major infected mice on Balb/c background after 6 weeks. (b) Absolute numbers in ear of total MHCII^hiCD11c⁺ DCs; total CD4⁺CD3⁺ and CD8⁺CD3⁺ T cells, measured by flow cytometry. (c–e) dLN infiltration of cells. Absolute numbers in dLN of total MHCII^hi DCs; total CD4⁺CD3⁺ and CD8⁺CD3⁺ T cells; CD4⁺/CD8⁺ T-cell ratio; IFNγ⁺CD4⁺CD3⁺ and IFNγ⁺CD8⁺CD3⁺ cells, measured by flow cytometry. (a–e) Bar represents mean value and each dot represents one ear or dLNs. Results from week 2 and week 6 are representative of two separate experiments. WT control n=3–4; WKO control n=4; WT L. major 2 weeks n=6; WASp KO L. major 2 weeks n=6; WT L. major 6 weeks n=10; WASp KO L. major 6 weeks n=7. *P<0.05; **P<0.01 as calculated by the unpaired Student's t-test. FMO, fluorescence minus one (negative control for MHC class II and CD103); WT, wild type; WKO, WASp KO.