Table 2.
Details of the Stereologic Counting Procedures
| Subiculum | CA1 | CA2/3 | CA4 | DG | |
|---|---|---|---|---|---|
| Obj. | 50× | 50× | 50× | 50× | 50× |
| B (μm2) | 900 | 900 | 900 | 900 | 900 |
| H (μm) | 17 | 17 | 17 | 17 | 17 |
| D (μm) | 930×510 | 450×570 | 990×480 | 830×430 | 270×130 |
| ∑CS | 200 | 120 | 250 | 160 | 130 |
| ∑Q− neurons | 3260 | 2037 | 4851 | 3214 | 8907 |
| ∑Q− astrocytes | 270 | 178 | 317 | 230 | 172 |
| ∑Q− oligodendrocytes | 1187 | 464 | 1053 | 734 | 296 |
| CEpred (neurons) | 0.049 | 0.048 | 0.046 | 0.05 | 0.033 |
| CEpred (astrocytes) | 0.051 | 0.049 | 0.053 | 0.048 | 0.050 |
| CEpred (oligodendroc.) | 0.038 | 0.042 | 0.048 | 0.039 | 0.041 |
Note: Obj, objective used; B and H, base and height of the unbiased virtual counting spaces; D, distance between the unbiased virtual counting spaces in mutually orthogonal directions x and y; ∑CS, average sum of unbiased virtual counting spaces used in one hemisphere of one proband; ∑Q− neurons, average number of counted neurons in one hemisphere of one proband; ∑Q− oligodendrocytes, average number of counted oligodendrocytes in one hemisphere of one proband; ∑Q− astrocytes, average number of counted astrocytes in one hemisphere of one proband; CEpred, average predicted coefficient of error of estimated cell numbers; Oligodendroc., oligodendrocytes; DG, dentate gyrus; CA, Cornu Ammonis.