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. 2016 Jul 26;6:30183. doi: 10.1038/srep30183

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(A) PNS was prepared from HuH7 cells expressing both ABCD4-HA and ABCD4-His. After treatment with 0.5% β-DDM, the assembly of ABCD4 was analyzed by BN-PAGE followed by immunoblotting with an anti-HA (left) or anti-His (right) antibody. (B) Photocrosslinking of ABCD4-HA was performed with SDA and UV irradiation. ABCD4-HA was detected by immunoblot analysis with an anti-HA antibody.