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. 2016 Jul 1;14(5):298–307. doi: 10.1089/adt.2016.728

Fig. 2.

Fig. 2.

Azithromycin elevates the number of non-inflammatory microglia/macrophages in the ipsilateral ischemic hemisphere of mice subjected to transient MCAo. (A) Representative immunofluorescence images showing expression of MAC-1 (green fluorescence) in the ipsilateral (Ipsi) and contralateral (Contra) striatum of mice subjected to 30-min MCAo followed by 48 h of reperfusion and injected with vehicle (VEH, saline, 1 mL/kg) or azithromycin (AZM, 150 mg/kg), i.p., upon reperfusion. Cell nuclei were counterstained with DAPI (red signal). (B) As compared with vehicle-injected animals, azithromycin elevates the number of MAC-1-immunopositive microglia/macrophages in the ipsilateral striatum. Scale bar: 100 μm. *P < 0.05 versus vehicle (two-tailed Student's t-test, n = 3 animals per experimental group). (C) Representative immunofluorescence images of the ischemic striatum showing colocalization of the microglia/macrophages marker MAC-1 with MPO, a marker of M1 polarization in vehicle-injected animals (VEH, saline 1 mL/kg, i.p., upon reperfusion), but not in mice treated with azithromycin (AZM, 150 mg/kg, i.p., upon reperfusion) subjected to 30-min MCAo followed by 48 h of reperfusion. Conversely, microglia/macrophages from mice treated with azithromycin were immunopositive for the M2 marker Ym1. Cell nuclei were counterstained with DAPI (blue signal). Scale bars: 100 μm. MPO, myeloperoxidase.