Figure 1. Dengue virus (DENV) infection induced IFN production in human DCs.
Human DCs (1 × 106 cells/ml) were infected by mock or different strains of DENV2, including NGC, 16681 and PL046 strains at a MOI of 5. Quantitative RT/PCR was used to determine expression of mRNAs of the IFN-λ1, -λ2, -λ3 and -β1 genes (A). Similarly, mRNA levels of these IFNs were determined in DCs infected by different subtypes of DENV (B). The mRNA levels of IFN-λ1 in DCs infected by the DENV2 NGC strain at different MOIs were measured (C). The protein levels of IFN-λ1 in supernatants collected from different conditions were determined by ELISA (D). The data shown are from various numbers of different donor DCs as indicated (n). The analysis was performed by ANOVA or Student’s t test. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001.