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. 2016 Jul 26;6:24530. doi: 10.1038/srep24530

Figure 3. DENV-induced IFN-λ1 expression was through NS1 in human lung epithelial cell line A549.

Figure 3

A549 cells were infected by DENV, and the mRNA levels of IFNs were determined (A). Transfection of various doses of plasmids encoding different components of viral NS genes (PCR3.1-NS-flag) into A549 cells (1 × 105 cells/ml) resulted in varying expression of viral NS proteins, as determined by Western blotting using equal amount of loading protein for analysis (B). Various amounts of plasmids containing viral NS genes corresponding to the equivalent viral NS proteins measured by Western blotting were transfected into A549 cells and the fold induction of mRNAs of IFNs was determined by RT/PCR (C). The expression of NS1 in human DCs infected by DENV for various time points was determined by Western blotting (D). The data show representative results and analysis pooled from three independent experiments examining different donor DCs. **p < 0.01.